Journal: ACS Omega

Article Title: Investigating the Mechanism of the Fuzheng Huayu Formula in Treating Cirrhosis through Network Pharmacology, Molecular Docking, and Experimental Verification

doi: 10.1021/acsomega.5c01225

Figure Lengend Snippet: Western blot analysis results. (A–C) Representative images of Western blot analysis of p38 MAPK, IKKβ, and NF-κB p65. (D–F) Relative phosphorylation levels of p38 MAPK, IKKβ, and NF-κB p65. Isotanshinone II dose-dependently inhibits phosphorylation of p38 MAPK, IKKβ, and NF-κB p65 in TGF-β1-induced LX-2 cells. Data represent mean ± SD ( n = 3). * p < 0.05, ** p < 0.01, *** p < 0.001 vs the TGF-β group.

Article Snippet: Membranes were blocked with 5% skim milk for 2 h and incubated overnight at 4 °C with the following primary antibodies: polyclonal rabbit antihuman TGF-β1 (1:1000, Boster Biological Technology, Wuhan, China), GAPDH (1:20,000, Boster Biological Technology, Wuhan, China), p38 MAPK (1:1000, Boster Biological Technology, Wuhan, China), p-p38 MAPK (1:1000, Boster Biological Technology, Wuhan, China), IKKβ (1:1000, Boster Biological Technology, Wuhan, China), p -IKKβ (1:2000, ABclonal, Wuhan, China), NF-κB (1:1000, Boster Biological Technology, Wuhan, China), and phospho–NF–κB p65(1:2000, Cell Signaling Technology, Danvers, MA, USA).

Techniques: Western Blot, Phospho-proteomics